nACh subunit a2

Click on sections of picture to learn more about research on individual amino acids. Numbering of the nACh a2 subunit sequences starts at the end of the signal sequence. Only amino acids in the mature protein are shown.


amino acid all M3 M2 M1-M2 Linker M1 M2-M3 Linker N-Terminus M3-M4 Linker M4

nACh a2 -- GENBANK

nACh a2 -- FASTA

nACh a2 -- Alignment

 

 

 

 

 

 

 

 

 

 

 

 

N-Terminus

Val 260 Arg 263 Arg 262 Ile 261 Thr 256 Ala 258 Tyr 257 Phe 259 Asp 254 Phe 253 Tyr 252 Ile 251 Glu 250 Ala 249 Cys 248 Cys 247 Asp 246 Tyr 245 Lys 244 Lys 243 Ser 242 Asn 241 Tyr 240 Thr 239 Gly 238 Thr 237 Val 255 Ala 236 Asn 235 Val 234 Ile 233 Ala 232 Trp 231 Glu 230 Gly 229 Ser 228 Glu 227 Trp 226 Tyr 225 Asp 224 Lys 223 Leu 222 Asp 221 Val 220 Thr 219 Gln 218 Glu 217 Met 216 Gln 215 Glu 214 Leu 213 Asp 212 Ile 211 Lys 210 Ala 209 Gln 194 Gln 195 Asn 196 Cys 197 Lys 198 Met 199 Lys 200 Phe 201 Gly 202 Ser 203 Trp 204 Thr 205 Tyr 206 Asp 207 Lys 208 Asp 193 Phe 192 Pro 191 Phe 190 Phe 189 Thr 188 Val 187 Asp 186 Ile 185 Ser 184 Cys 183 Ser 182 Ser 181 Lys 180 Tyr 179 Ile 178 Ala 177 Pro 176 Pro 175 Val 174 Trp 173 His 172 Val 171 Thr 170 Gly 169 Thr 168 Ser 167 Phe 166 Leu 165 Asn 149 Asn 150 Ala 151 Asp 152 Gly 153 Gln 154 Phe 155 Ala 156 Val 157 Thr 158 His 159 Met 160 Thr 161 Lys 162 Ala 163 His 164 Lys 119 Tyr 118 Asp 117 Ser 116 Trp 115 Glu 114 Gln 113 Lys 112 Leu 111 Trp 110 Val 109 Asn 108 Thr 107 Thr 106 Met 105 Met 104 Leu 120 Arg 121 Trp 122 Asn 123 Pro 124 Thr 125 Asp 126 Phe 127 Gly 128 Asn 129 Ile 130 Thr 131 Ser 132 Leu 133 Arg 134 Val 135 Pro 136 Ser 137 Glu 138 Met 139 Ile 140 Trp 141 Ile 142 Pro 143 Asp 144 Ile 145 Val 146 Leu 147 Tyr 148 Gln 103 Asn 102 Lys 101 Glu 100 Asp 99 Val 98 Asp 97 Ile 96 Leu 95 Gln 94 Ala 93 Ile 92 Ser 91 Leu 90 Gly 89 Phe 88 Arg 87 Val 86 Ile 85 Val 84 Val 83 Asp 82 Ser 81 Thr 80 Asn 79 Pro 78 Val 77 Pro 76 Arg 75 Glu 59 Asp 60 Arg 61 Leu 62 Phe 63 Lys 64 His 65 Leu 66 Phe 67 Arg 68 Gly 69 Tyr 70 Asn 71 Arg 72 Trp 73 Ala 74 Pro 33 Pro 32 Arg 31 Lys 30 Ala 29 Glu 28 Glu 27 Pro 34 Arg 35 Ala 36 Pro 37 Gly 38 Asp 39 Pro 40 Leu 41 Ser 42 Ser 43 Pro 44 Ser 45 Pro 46 Thr 47 Ala 48 Leu 49 Pro 50 Gln 51 Gly 52 Gly 53 Ser 54 His 55 Thr 56 Glu 57 Thr 58

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M1

Tyr 288 Leu 289 Phe 287 Pro 290 Phe 267 Leu 266 Pro 265 Leu 264 Phe 267 Leu 264 Pro 265 Leu 266 Thr 283 Leu 285 Val 286 Val 284 nACh/a2_C226.htm Leu 282 Cys 281 Ser 280 Ile 279 Leu 278 Leu 277 Cys 276 Pro 275 Ile 274 Ile 273 Leu 272 Asn 271 Ile 270 Thr 269 Tyr 268

Residues with mutations: [ Ile 279 ] Arg 1 Ala 2 His 3 Ala 4 Glu 5 Glu 6 Arg 7 Leu 8 Leu 9 Lys 10 Lys 11 Leu 12 Phe 13 Ser 14 Gly 15 Tyr 16 Asn 17 Lys 18 Trp 19 Ser 20 Arg 21 Pro 22 Val 23 Ala 24 Asn 25 Ile 26 Ser 27 Asp 28 Val 29 Val 30 Leu 31 Val 32 Arg 33 Phe 34 Gly 35 Leu 36 Ser 37 Ile 38 Ala 39 Gln 40 Leu 41 Ile 42 Asp 43 Val 44 Asp 45 Glu 46 Lys 47 Asn 48 Gln 49 Met 50 Met 51 Thr 52 Thr 53 Asn 54 Val 55 Trp 56 Val 57 Lys 58 Gln 59 Glu 60 Trp 61 His 62 Asp 63 Tyr 64 Lys 65 Leu 66 Arg 67 Trp 68 Asp 69 Pro 70 Ala 71 Asp 72 Tyr 73 Glu 74 Asn 75 Val 76 Thr 77 Ser 78 Ile 79 Arg 80 Ile 81 Pro 82 Ser 83 Glu 84 Leu 85 Ile 86 Trp 87 Arg 88 Pro 89 Asp 90 Ile 91 Val 92 Leu 93 Tyr 94 Asn 95 Asn 96 Ala 97 Asp 98 Gly 99 Asp 100 Phe 101 Ala 102 Val 103 Thr 104 His 105 Leu 106 Thr 107 Lys 108 Ala 109 His 110 Leu 111 Phe 112 His 113 Asp 114 Gly 115 Arg 116 Val 117 Gln 118 Trp 119 Thr 120 Pro 121 Pro 122 Ala 123 Ile 124 Tyr 125 Lys 126 Ser 127 Ser 128 Cys 129 Ser 130 Ile 131 Asp 132 Val 133 Thr 134 Phe 135 Phe 136 Pro 137 Phe 138 Asp 139 Gln 140 Gln 141 Asn 142 Cys 143 Thr 144 Met 145 Lys 146 Phe 147 Gly 148 Ser 149 Trp 150 Thr 151 Tyr 152 Asp 153 Lys 154 Ala 155 Lys 156 Ile 157 Asp 158 Leu 159 Val 160 Asn 161 Met 162 His 163 Ser 164 Arg 165 Val 166 Asp 167 Gln 168 Leu 169 Asp 170 Phe 171 Trp 172 Glu 173 Ser 174 Gly 175 Glu 176 Trp 177 Val 178 Ile 179 Val 180 Asp 181 Ala 182 Val 183 Gly 184 Thr 185 Tyr 186 Asn 187 Thr 188 Arg 189 Lys 190 Tyr 191 Glu 192 Cys 193 Cys 194 Ala 195 Glu 196 Ile 197 Tyr 198 Pro 199 Asp 200 Ile 201 Thr 202 Tyr 203 Ala 204 Phe 205 Val 206 Ile 207 Arg 208 Arg 209 Leu 210 Pro 211

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M1-M2 Linker

Lys 296 Glu 295 Ser 291 Asp 292 Cys 293 Gly 294

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Gly 294

M2


Leu 299 Ile 297 Pro 319 Ile 318 Thr 298 Val 303 Ser 302 Ile 301 Cys 300 Leu 304 Leu 305 Ser 306 Leu 307 Thr 308 Val 309 Phe 310 Leu 311 Leu 312 Leu 313 Ile 314 Thr 315 Glu 316 Ile 317

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M2-M3 Linker

Ser 320 Thr 321 Ser 322 Leu 323 Val 324 Ile 325

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M3

Tyr 331 Glu 330 Gly 329 Ile 328 Leu 327 Leu 332 Pro 326 Leu 333 Phe 334 Thr 335 Met 336 Ile 337 Phe 338 Val 339 Ile 340 Leu 341 Ser 342 Ile 343 Val 344 Ile 345 Thr 346 Val 347 Phe 348 Val 349

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M3-M4 Linker

Val 352 Asn 351 Leu 350 His 353 His 354 Arg 355 Ser 356 Pro 357 Ser 358 Thr 359 His 360 Thr 361 Met 362 Pro 363 His 364 Trp 365 Val 366 Arg 367 Gly 368 Ala 369 Leu 370 Leu 371 Gly 372 Cys 373 Val 374 Pro 375 Arg 376 Trp 377 Leu 378 Leu 379 Met 380 Ile 319 Val 320 Pro 321 Arg 322 Leu 323 Leu 324 Leu 325 Met 326 Lys 327 Arg 328 Pro 329 Ser 330 Val 331 Val 332 Lys 333 Asp 334 Asn 335 Cys 336 Arg 337 Arg 338 Leu 339 Ile 340 Glu 341 Ser 342 Met 343 His 344 Lys 345 Met 346 Ala 347 Ser 348 Ala 349 Pro 350 Arg 351 Phe 352 Trp 353 Pro 354 Glu 355 Pro 356 Glu 357 Gly 358 Glu 359 Pro 360 Pro 361 Ala 362 Thr 363 Ser 364 Gly 365 Thr 366 Gln 367 Ser 368 Leu 369 His 370 Pro 371 Pro 372 Ser 373 Pro 374 Ser 375 Phe 376 Cts 377 Val 378 Pro 379 Leu 380 Asp 381 Val 382 Pro 383 Ala 384 Glu 385 Pro 386 Gly 387 Pro 388 Ser 389 Cys 390 Lys 391 Ser 392 Pro 393 Ser 394 Asp 395 Gln 396 Leu 397 Pro 398 Pro 399 Gln 400 Gln 401 Pro 402 Leu 403 Glu 404 Ala 405 Glu 406 Lys 407 Ala 408 Ser 409 Pro 410 His 411 Pro 412 Ser 413 Pro 414 Gly 415 Pro 416 Cys 417 Arg 418 Pro 419 Pro 420 His 421 Gly 422 Thr 423 Gln 424 Ala 425 Pro 426 Gly 427 Leu 428 Ala 429 Lys 430 Ala 431 Arg 432 Ser 433 Leu 434 Ser 435 Val 436 Gln 437 His 438 Met 439 Ser 440 Ser 441 Pro 442 Gly 443 Glu 444 Ala 445 Val 446 Glu 447 Gly 448 Gly 449 Val 450 Arg 451 Asn 381 Arg 382 Pro 383 Pro 384 Pro 385 Pro 386 Val 387 Glu 388 Leu 389 Cys 390 His 391 Pro 392 Leu 393 Arg 394 Leu 395 Lys 396 Leu 397 Ser 398 Pro 399 Ser 400 Tyr 401 His 402 Trp 403 Leu 404 Glu 405 Ser 406 Asn 407 Val 408 Asp 409 Ala 410 Glu 411 Glu 412 Arg 413 Glu 414 Val 415 Val 416 Val 417 Glu 418 Glu 419 Glu 420 Asp 421 Arg 422 Trp 423 Ala 424 Cys 425 Ala 426 Gly 427 His 428 Val 429 Ala 430 Pro 431 Ser 432 Val 433 Gly 434 Thr 435 Leu 436 Cys 437 Ser 438 His 439 Gly 440 His 441 Leu 442 His 443 Ser 444 Gly 445 Ala 446 Ser 447 Gly 448 Pro 449 Lys 450 Ala 451 Glu 452 Ala 453 Leu 454 Leu 455 Gln 456 Glu 457 Gly 458 Glu 459 Leu 460 Leu 461 Leu 462 Ser 463 Pro 464 His 465 Met 466 Gln 467 Lys 468 Ala 469 Leu 470 Glu 471 Gly 472 Val 473 His 474 Tyr 475 Ile 476 Ala 477 Asp 478 His 479 Leu 480 Arg 481 Ser 482 Glu 483 Asp 484 Ala 485 Asp 486 Ser 487 Ser 488 Val 489 Lys 490 Glu 491 Asp 492 Trp 493 Lys 494

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M4

Val 496 Tyr 495 Ile 529 Met 528 Gly 528 Ile 517 Leu 519 Phe 524 Pro 523 Pro 522 Gly 527 Ala 526 Leu 525 Ala 497 Met 498 Arg 502 Ile 503 Val 499 Ile 500 Asp 501 Phe 504 Leu 505 Ile 509 Ile 510 Trp 506 Leu 507 Phe 508 Val 511 Cys 512 Phe 513 Leu 514 Gly 515 Thr 516 Gly 518 Phe 520 Leu 521

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Alignment